Postembedding immunocytochemistry of large sections of brain tissue: an improved flat-embedding technique
نویسندگان
چکیده
منابع مشابه
Postembedding immunocytochemistry of large sections of brain tissue: an improved flat embedding technique.
A method for osmicating, dehydrating, and flat-embedding large slabs of brain tissue in epoxy resin is presented. This permits the production of semithin sections for postembedding immunocytochemistry that are far larger than can be obtained with other embedding approaches. Vibratomed slabs, 50-200 microns thick and as large as 6 x 8 mm are embedded in a 'soft' Araldite epoxy. The slabs are lam...
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AIMS To investigate the effect of tissue preparation on immunostaining and to establish whether there is a standard tissue preparation schedule that allows optimal demonstration of all antigens. METHODS Blocks of tonsil were subjected to variations to a standard fixation, processing, and section preparation schedule. The sections were stained with five antibodies-L26 (CD20), UCHL1 (CD45RO), C...
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ژورنال
عنوان ژورنال: Journal of Neuroscience Methods
سال: 1996
ISSN: 0165-0270
DOI: 10.1016/0165-0270(95)00111-5